Mathematical models can be used to explore tick-borne pathogen dynamics, quantify risk of tick-borne disease and identify optimal strategies to reduce that risk. For the past four years, questing ticks have been collected from ten separate locations in the southeastern portion of Virginia using a variety of techniques. These data were then used to estimate parameters for an agent-based mathematical model. This study helped to identify times and places with high risk of tick-borne diseases to humans today and in the future.